Ref ID : 3950
M.J. Sinclair, J.F. McClellan, J.L. Sinclair, and D.C. Coleman; Yield Constant for Acanthamoeba polyphaga in Batch and Continuous Culture. J.Protozool. 28(4):479-483, 1981
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A two-stage chemostat modified to accommodate the growth of adhesive organisms was used to determine the yield constant, Y, of a representative soil amoeba, Acanthamoeba polyphaga, utilizing as its prey Pseudomonas paucimobilis. The first stage consisted of a glucose-limited bacterial culture in steady state. The second stage consisted of a simplified predator-prey system, nongrowing bacteria serving as the limiting substrate for amoebae. A refined methodology to more accurately determine Y was developed, and Y for Acanthamoeba polyphaga in batch and continuous culture was determined to be 19.1%.