Ref ID : 3839
M. Bourdeau, G. Labonte, M. Ahmad, and P. Couillard; The Contractile Vacuole of Amoeba proteus. II. Numerical analysis of the Steady-State Hypothesis. J.Protozool. 25(4):481-485, 1978
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At temperature below 15 degrees C, the contractile vacuole cycle of Amoeba proteus includes a presystolic plateau. The hypothesis attributing this plateau to a steady-state equilibrium between active filling processes and osmotic losses of water from the vacuole into the cytoplasm has been expressed in an equation predicting vacuolar diameter as a function of time for the later part of the cycle. Computer-generated model cycles have been compared with actual recorded cycles at 15 degrees C, 10 degrees C, and 5 degrees C and conditions of best fit were determined. Statistical analysis shows that recorded cycles are quite compatible with the steady-state hypothesis.