Ref ID : 3794
Helen G. Hansma; The immobilization Antigen of Paramecium aurelia is a Single Polypeptide Chain. J.Protozool. 22(2):257-259, 1975
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The immobilization antigen (i-antigen) fraction of Paramecium aurelia syngen 4 is shown to contain a protease that is activated by mercaptoethanol. After the protease has been heat-inactivated, the molecular weight of the i-antigen (~250,000 daltons) cannot be decreased by mercaptoethanol treatment. It is demonstrated that the i-antigen is a single polypeptide chain. Reasons are also given why low molecular weight subunits were previously reported by other authors.