Ref ID : 3706
Jacques Bohatier, Francoise Bernard, and Francois Amalric; Electrophoretic Study of Newly-synthetisized RNA during Oral Regeneration of the Heterotrichous Ciliate Protozoan Stentor coeruleus. Arch.Protistenk 127:131-150, 1983
Reprint
In File
Notes
An electrophoretic study was made of RNA newly-formed during two periods of preferential [3H]-uridine uptake in Stentor undergoing traumatic regeneration of oral apparatus. Three main categories of RNA were detected during both periods; namely, ribosomal RNAs 18S and 28S; low molecular weight RNAs 4-6S and polydispersed RNAs 7S-22S. Among the latter, an RNA 7-8S was detected in large amounts during both periods, and an RNA 16-18S appeared in the second period. This last RNA was also found in total RNA for the whole regeneration cycle (8 hr) and in total RNA from dividing intact cells (16 hr). The percentage of oligo(dT)-cellulose bound RNA, always low, was highest at early stages. Electrophoretic analysis showed that most of the new species were poly A-. Although poly A+ RNAs are preferentially synthesized at the beginning of morphogenesis, the existence of poly A- messengers in Stentor cannot be excluded.