Ref ID : 3610
W. Davies, K.S. Jakobsen, and O. Nordby; Characterization of DNA from the Dinoflagellate Woloszynskia bostoniensis. J.Protozool. 35(3):418-422, 1988
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Some physical and chemical properties of DNA isolated from the dinoflagellate Woloszynskia bostoniensis were determined. Analytical cesium chloride gradient centrifugation gave a major component and a minor component banding at 1.719 and 1.693 g/cm, respectively. Thermal denaturation in 0.1 SSC showed a broad transition with a Tm of 70.5 degrees C. Derivation of this curve indicated that two components were present having Tm values of 66 degrees C and 70 degrees C. Base composition analysis showed a GC content of 48.1% and a high degree of thymine replacement by 5-hydroxymethyluracil. Two minor bases, identified as 5-methylcytosine and N6-methyladenine, were also detected. Reassociation kinetics showed a typical eukaryotic reassociation pattern with 45% repetitive and 55% single copy sequences.