Ref ID : 2387
Fukusho Kunihiko, Okauchi Masanori, Tanaka Hideki, Prakit Kraisingdecha, Sri Indar Wahyuni, and Watanabe Takeshi; [Food Value of the small S-strain of a rotifer Brachionus plicatilis cultured with Tetraselmis tetrathele for Larvae of Black Sea Bream Acanthopagrus shlegeli]. Bull.Natl.Res.Inst.Aquaculture 8:5-13, 1985
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A minute alga Tetraselmis tetrathele is 20-30 times larger than Chlorella sp. in cell volume. However, T. tetrathele was found to be suitable as food organism for the small strains (S-strain) of rotifer, Brachionus plicatilis rotundiformis as well as the large strain (L-strain) B. p. typicus, although the dietary value of S-strain cultured with T. tetrathele remains unknown. The dietary value of S-strain of rotifers cultured with T. tetrathele (TR) or both T. tetrathele and Chlorella sp. (TCR) was compared with those cultured with Chlorella sp. (CR) by feeding these various rotifers to larval black sea bream Acanthopagrus schlegeli for 15 days. The rotifers enriched by Chlorella (12-24 hours) after culturing with T. tetrathele (TR-C) were also given to the larvae. The survival rate, total length, and body weight of fish fed TR were inferior to those of the fish receiving CR (35.9 and 57.3% vs. 93.5 and 94.6% in survival rate, 9.8 and 10.2 mm vs. 11.1 and 11.4 mm in T. L., 5.9 and 5.9 mg vs. 8.7 and 9.8 mg in body weight). However, the survival rate and the growth of fish fed TCR or TR-C were superior to fish fed with TR., i.e., 90.9-93.9% , 11.3-11.5 mm, and 10.1-10.4 mg in TCR; 84.8-96.9%, 11.3-12.0 mm, and 9.8-12.4 mg in TR-C. These differences among the larvae fed rotifers with different treatments might be due to difference in the content of w3HUFA in rotifers. The content of w3HUFA in TR, TCR, TR-C, and CR was 0.21, 0.76, 0.66 and 0.80% respectively. Thus, the amount of w3HUFA in TR was not enough for larval black sea bream judging from the w3HUFA requirement of larval red sea bream Pagrus major. The dietary value was effectively improved due to incorporation of w3HUFA by combination or secondary culture (24 hr) with Chlorella sp. The poor dietary value of TR used in this experiment may partly be due to a lower lipid accumulation in TR than rotifers with other treatments.