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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 1596

Laura K. King and Bruce C. Parker; A simple, rapid method for enumerating total viable and metabolically active bacteria in groundwater. Appl.Environ.Microbiol. 54(6):1630-1631, 1988

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We report here a new staining procedure which uses both the enzymatic dehydrogenation of 2-(p-iodophenyl)-3-p-(nitrophenyl)-5 phenyltetrazolium chloride to a pink intracellular formazan and the DNA-specific fluorochrome 4',6'-diamidino-2-phenylindole. Application of this staining procedure to cells concentrated on filters and then transferred to microscope slides by the filter-transfer-freeze technique has proven valuable for statistically accurate enumeration of the total viable and metabolically active cells in groundwaters.