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:2017/08/25 16:49:59

TIN-TIN-WIN-SHWE

TIN-TIN-Win-Shwe

TIN-TIN-WIN-SHWE
氏名
TIN-TIN-WIN-SHWE(てぃんてぃん うぃん しゅい)
所属/職名
環境リスク・健康研究センター(生体影響評価研究室)/主任研究員
研究課題
Analysis of developmental neurotoxicity of environmental chemicals in mice
学位の種類
M.D., Ph.D (Medical Science)
専門とする学問分野
医学,生理学,生化学
専門とする環境分野
hazardous chemical substance,atmospheric pollutants,volatile organic compounds
自分の強みのキーワード
in vivo microdialysis in rats and mice,HPLC assay for neurotransmitter assessment,Radioimmunoassay for hormone measurement,Gonadectomization for male and female rats,Intracardiac catheterization and blood sampling,Guide cannula implatation in various brain regions,Dissection and sampling of various brain regions,RNA isolation, DNA synthesis and real-time PCR,Learning behavior assessment by Morris water maze ,Brain perfusion and section using microtome
関心のある研究、実施してみたい研究
My principal research interest is Neurotoxicology, particularly studying the effects of environmental chemicals on higher brain functions by measuring neurotransmitter levels in a freely moving animal model using in vivo microdialysis and high performance liquid chromatography assay. Then, I am interested to examine the effects of these environmental chemicals on learning performance and relevant gene expression in adults and developmental exposure models. Major focuses are to examine the effects of exposure to volatile organic compound like toluene, nanoparticles and organophosphate pesticides on neurotransmitter levels, memory function-related gene expressions in the hippocampus and learning ability in a mouse model.
研究概要
I have studied the effects of environmental toxic chemicals like volatile organic compounds (VOC) and environmental pollutants like nanoparticles on brain neurotransmitter system and memory-function related gene expression in adult mouse hippocampus and olfactory bulb. I have demonstrated neurotoxic effects of toluene exposure in BALB/c adult mice and two congenic adult mice strains by measuring excitatory amino acid neurotransmitter levels in the hippocampus (Win-Shwe et al., 2007a; 2009). Moreover, I have also shown that the effect of toluene exposure on N-methyl-D-aspartate (NMDA) receptor subunit expression in hippocampus of wild type and nude mice (Win-Shwe et al., 2007c). Regarding nanoparticles, I have already reported that the effects of nanoparticle-rich diesel exhaust on spatial learning and memory function-related gene expression in the mouse hippocampus (Win-Shwe et al., 2008) and on extracellular glutamate level and NMDA receptor subunit expression in the mouse olfactory bulb (Win-Shwe et al., in press). However, I have not examined the developmental neurotoxicity of these substances yet, then, up to now, the mechanism by which toluene or nanoparticle exposure during the brain growth spurt affected cognitive functions and glutamate toxicity are unknown. These situations prompt me to examine the effect and mechanism of toluene or nanoparticle exposure during developmental period with a new novel experimental design. Recently, serious public problems concerning food contamination with agrichemicals occurred in Japan and as where. Organophosphate pesticides (OPs) are used worldwide especially South-East Asia including Myanmar. Among OPs, diazinon is used in order to combat insects and arthropods in agricultural industries. Children may be exposed to that OP by getting the residues on foods or by contacting the contaminated surfaces in houses and gardens. However, there is no study reporting the effects of developmental exposure to diazinon on glutamatergic neurotransmitter system in in vivo experiments. Therefore, using our new experimental model, I emphasize to examine the developmental neurotoxicity following exposure to diazinon.
The current aims of research are to investigate the effect of prenatal and/or
postnatal exposure to nanoparticles, diazinon and toluene on glutamatergic
neurotransmission, related gene expression and learning ability in adult mice and to investigate the possible mechanisms of these three substances. Microdialysis is a novel technique allowing an in vivo measurement of endogenous and exogenous substances in the extracellular fluid surrounding a microdialysis probe implanted into an organ or tissue. Hippocampus is important for spatial memory, but its integrity is also necessary for recognition memory. Novel object recognition task relies on the mouse’s natural exploratory behavior and it can be used without stress, technical difficulty and time consuming. Therefore, in the present research, I try to combine these two techniques to establish an experimental model to examine extracellular neurotransmitters and learning ability simultaneously. I expect to measure the extracellular glutamate levels in the hippocampus of adolescent and adult mice while monitoring learning ability in sequentially in different doses following prenatal and/or postnatal exposure to environmental toxicant. Then, I can point out the vulnerable period or critical window for susceptibility of individuals to toxic chemical exposure. I can evaluate the relationship between neurotransmitter system and learning performance. I can also find out the possible mechanism of action of environmental toxicant by using receptor blocker or measuring transporter level. After establishment of this new experimental design, it can be used for assessing the other environmental toxic chemicals in various brain regions for examining higher brain functions.
略歴
4/1/1979−3/25/1986---Institute of Medicine 1, Yangon, Myanmar [M. D] (Completion of Medicine, Surgery, Pediatric, Obstetric and Gynecology)
1/4/1987−31/3/1990---Medical Research Officer, WHO project, “Study of the effects of water supply, socio-economic status and sanitation on health in Myanmar rural areas”, Department of Medical Research, Ministry of Health, Yangon, Myanmar
1/4/1990−31/3/1995---Assistant Lecturer, Department of Physiology, Institute of Medicine 1, Yangon, Myanmar
4/1/1992−3/25/1995---Institute of Medicine 1, Yangon, Myanmar [M. Med. Sc] Department of Physiology, Institute of Medicine 1, Yangon, Myanmar
1/4/1995−31/3/1998---Lecturer, Department of Physiology, Institute of Medicine 1, Yangon, Myanmar
4/1/1999−3/31/2003---Yokohama City University Graduate School of Medicine, Department of Physiology, [Ph. D]
1/11/2003−31/3/2006---Post-doctoral Fellow, Department of Health Sciences, NIES
1/4/2006−31/3/2008---JSPS Post-doctoral Fellowship for Foreign Researchers, Research Center for Environmental Risk, NIES
1/4/2008−30/3/2011---Research Fellow, Research Center for Environmental Risk, NIES
1/4/2011-up to present---Researcher, Center for Environmental Health Sciences, NIES

所属学会
日本神経科学学会,American Society for Neuroscience,日本免疫毒性学会,大気環境学会,日本トキシコロジー学会
個別研究課題
研究成果(誌上)
  • all
  • 査読付き 原著論文
  • 総説・解説
  • 書籍
  • その他
  • Environmental Chemical Substances in Relation to Neurodevelopmental Disorders: A Systematic Literature Review
    発表者 : Sone H.(曽根秀子), Win-Shwe T.T.(Tin-Tin-Win-Shwe), Qin X.Y.(QIN XIANYANG), Akanuma H.(赤沼宏美), Imanishi S.(今西哲)
    掲載誌 : Learning Disabilities (2012)
研究成果(口頭)
  • all
  • 研究発表
  • 研究講演
所の刊行物
委員会活動
  • 2016年度 : 室内環境学会委員会理事 ((一社)室内環境学会)
  • 2014年度 : [連携]連携大学院方式に係る教員(教授)(持続環境学フォーラムI、等) (筑波大学大学院生命環境系)
受賞
  • 2014年 : 年会賞 (日本免疫毒性学会)
学会活動
  • 2016年度 : 日本免疫毒性学会 : 平成28年度日本免疫毒性学会評議員
  • 2015年度 : 日本免疫毒性学会 : 平成27年度日本免疫毒性学会評議員
  • 2014年度 : 日本免疫毒性学会 : 平成26年度日本免疫毒性学会評議員
  • 2013年度 : 日本免疫毒性学会 : 平成25年度日本免疫毒性学会評議員
  • 2012年度 : 日本免疫毒性学会 : 平成24年度日本免疫毒性学会評議員