Stylonychia
- Stylonychia lemnae
- Stylonychia notophora
- Stylonychia pustulata
[ref. ID; 4390]
Test system
Isolation of Cadmium-binding protein
Toxicant/concentrations
Final Cd concentration 2.5 uM Cd (0.28 ug Cd/ml).
Measurements/observations
Amino acid analysis.
[ref. ID; 483]
Test system
The synthesis of RNA and proteins
Strains
Food: Klebsiella aerogenes
Toxicant/concentrations
DDT 100 ppm for 6 hr.
Test design
Temperature 22+/-1 degrees C. Chalkley's medium (25 ciliate of the same age group).
RNA synthesis by using [3H]-uridine (20 uCi/ml), and protein synthesis by using [3H]-lysine (5 uCi/ml).
Measurements/observations
Grain counts were made from 100 areas (100 um2 per area) over the nucleus and cytoplasm separately.
Evaluations
The grain counts/unit area (100 um2).
[ref. ID; 4390]
Test system
Isolation of Cadmium-binding protein
Strains
From Prof. G.R. Sapra, Delhi, India.
Test concentrations
Final Cd concentration 15 uM Cd (1.68 ug Cd/ml).
Measurements/observations
Amino acid analysis.
[ref. ID; 6111]
Test system
Acute toxicity (24-hr LC50)
Strains
The organisms was obtained, initially, from Stirone Stream (northern Italy), a freshwater environment free from known toxicants and, in particular, from heavy metals. The culture medium was constituted of boiled rice and wheat grain in 10 ml of filtered natural water. 20+/-1 degrees C, photoperiod of 16:8 hr light:dark.
Toxicant/concentrations
NiCl2/6H2O (0.83, 1.12, 1.25, 1.4 ppm).
Test design
Tissue culture plates with 24 wells, 3 replicates.
Measurements
Mortality or the survivorship.
Evaluations
LC50 using the probit method.