Colpidium
- Colpidium campylum
- Colpidium colpoda
[ref. ID; 939]
Test system
Strains
Toxicants
CuSO4/5H2O, CdCl2, Ferbam and these mixture.
Test concentrations
Concentrations 0-15-30 ug/liter for Cu2+ and Cd2+ and at 0-150-300 ug/liter for ferbam.
Evaluation
Generations number by polynomial model.
[ref. ID; 6011]
Test system
Acute toxicity (24-hr LC50)
Strains
From the aeration tank of activated sludge works designated for the treatment of domestic wastes in the district of Reggio Emilia, northern Italy.
Toxicants
Hydrated cadmium chloride, hydrated copper chloride, zinc chloride, mercury chloride.
Test design
Costar tissue culture plates with 24 wells were employed. Two replicates of 12 organisms each were run of each test concentration.
Evaluations
LC50 using the probit method.
[ref. ID; 6081]
Test system
Strains
Toxicants
Co(NO3)2, NaF, Ni(NO3)2, SeO2, AsO3Na, MoO3Na, CrO4K2, Cr2O7K2, MnO4K, MnCl2, LiNO3, HgCl2, ZnSO4, Cd(NO3)2, CuSO4, PbCH3CO, KCN, SCNK, Fe(CN6K3), C6H5OH, C2H5OH.
Measurements
Number of cells using Coulter Counter.
Evaluations
Minimal Toxic Dose (DMA).
[ref. ID; 6784]
Test system
Acute toxicity
Strains
Monoxenically culture (Escherichia coli).
Toxicants
Aldrin, Azinphos-ethyl, Azinphos-methyl, Biphenyl, Bromophos-ethyl, Bromophos-methyl, Carbaryl, Dieldrin, Dimethoate, 2,4-D, 2,4'-DDD, 4,4'-DDD, 4,4'-DDE, 2,4'-DDT, 4,4'-DDT, alpha Endosulfan, beta Endosulfan, endrin, Fenchlorphos, Fenitrothion, Fenuron, HCB, Heptachlor, Heptachlorepoxid, Hexachlorophene, Lindane, MCPA,MCPB, Malathion, Methoxychlor, Paraoxon, Ethylparathion, Methylparathion, PCB2, PCB4, Pentachlorophenol, 2-Phenylphenol, Quintozen, 2,4,5-T, Thiram, Trichlorphon.
Test design
50 ml culture medium + 0.25 ml pesticide-acetone solution, 20 degrees C, incubation period 43 hr.
Measurements
Cell number using by Coulter Counter.
Evaluations
Minimal active dose (MAD).
[ref. ID; 7060]
Test system
Bioassay protocol (Influence of the initial cell concentration and intercalibration experiments between two laboratories)
Strains
Toxicants
K2Cr2O7, CdSO4, NaBO2, HgCl2, ZnCl2, Acrylnitril, Anilin, Cetrimide (cetyl-trimethylammonium bromide), Tetrapropylbenzene sulfonate (T.P.B.S.), Phenol.
Test design
The test is performed routinely in 30 ml crystal polystyrene screw-capped vials. If products tested can adsorb on plastic or alter it, borosilicate glass or teflon vials can be used. The vials are prepared for the test as follows:
- Toxicant solution in MM (MM medium composition:
CaCl2/2H2O 107 mg, NaCl 14.5 mg, NaNo3 4.5 mg, MgSO4/7H2O 75.7 mg, Na2SO4 39.5 mg, NaHCO3 135 mg, distilled, de-ionized or Milli-Q grade water added to 1000 ml, pH 8.15+0.02) 4 ml. 1.25 final concentration in the vial.
- Escherichia coli 0.25 ml. Strain ATCC 11303 (Sigma EC11303), strain ATCC 9637 (Sigma EC9637) or strain K12 (Sigma EC1), suspension 2.5 mg/ml in MM medium.
- C. campylum dilution, 3333 cells/ml 0.75 ml. The final volume is 5 ml and initial cell concentration is 500 cells/ml.
The vials are incubated in the dark at 28 degrees C for 24 hr. At the end of the incubation, each flask is fixed with 1 ml of 5% glutaraldehyde solution.
Measurements/observations
Cell density.
Evaluations
EC50.
[ref. ID; 7794]
Test system
Standard protocol proposal for growth inhibition test
Test design
Bioassay methodology.
Evaluations
24-hr IC50.
[ref. ID; 6111]
Test system
Acute toxicity (24-hr LC50)
Strains
The organisms was obtained, initially, from Stirone Stream (northern Italy), a freshwater environment free from known toxicants and, in particular, from heavy metals. The culture medium was constituted of boiled rice and wheat grain in 10 ml of filtered natural water. 20+/-1 degrees C, photoperiod of 16:8 hr light:dark.
Toxicant/concentrations
NiCl2/6H2O (1.12, 1.25, 1.4, 1.5 ppm).
Test design
Tissue culture plates with 24 wells, 3 replicates.
Measurements
Mortality or the survivorship.
Evaluations
LC50 using the probit method.