Amoeba proteus
[ref. ID; 485]
Test system
Acute assay and Cd-binding protein (Cd-thionein) assay
Strains
P(da)X(69).
Toxicants
[109]Cd2+
Test design
Modified Chalkley's solution (MCS), pH 5.9-6.0. Temperature 20+/-1 degrees C.
Measurements/observation
Survival, [109]Cd distribution.
Evaluations
LD80, Cd-binding protein.
[ref. ID; 5947]
Test system
Effect of inhibitor on the contractile vacuole
Strains
Originally obtained from the General Biological Supply House, Chicago (The amoebae were fed with the ciliate Tetrahymena pyriformis, which was provided by the Carolina Biological Supply House, Burlington, NC.)
Temperature
20 degrees C.
Inhibitor & concentrations
Sodium cyanide (short-term effects 15-20 min: 10E-4 M, long-term effects 3, 6, 9 hr: 3x10E-4 M), sodium malonate (3 hr: 5x10E-4 M), sodium succinate (3 hr: 5x10E-4M).
Measurements/observations
Contractile vacuole volume.