Eudrilus eugeniae
[ref. ID; 2173]
Test system
48-hr sublethal effect
Strains
From a local population bred from stock obtained from West Africa, via Prof. Graff of Braunschweig, Germany: Clitellate worms of between 100 and 120-days old.
Toxicants
Dieldrin
Test design/concentrations
The contact filter paper method, 25 degrees C, darkness, concentration (0.154, 0.462, 0.77 and 1.54 ug per cm2 of filter paper).
Measurements/observations
Sperm ultrastructure.
[ref. ID; 7159]
Test system
Contact test (48 hr) and aritifical soil test (2 weeks)
Strains
Toxicants
Carbaryl, 1,2-Dichloropropane, Dimethyl phthalate, Fluorene, Nitrobenzene, N-nitrosodiphenylamine, 4-Nitrophenol, Phenol, 1,2,4-Trichlorobenzene, 2,4,6-Trichlorophenol.
Test design
- Contact test: The glass vials used (Wheaton, 3 oz. snap cap) were 8 cm long and 3 cm in diameter. Strips of filter paper 12x6.7 cm (Whatman No.1) lined and completely covered the sides of the vial. One milliliter of water was added to the filter paper to provide enough moisture for earthworm survival. One adult earthworm was added per vial and the vials were kept on their side at 20 degrees C in a darkened incubator for 48 h. At least five concentrations were evaluated in the definitive test, with 10 or more replicates used for each concentration tested.
- EEC aritifical soil test: The medium consists by weight of 10% finely ground sphagnum peat, 20% kaolinite clay, 69% fine sand and 1% pulverized calcium carbonate. pH 6.0+/-0.5. water content 35% of the dry wt of the four components. Ten adult earthworms were added to 400 g (dry wt) of the test mixture in covered glass dishes 6.5 cm high and 12.5 cm in diameter. The dishes were incubated at 20 degrees C and mortality was assessed after 2 weeks. This test had at least five chemical concentration and four control dishes, all containing 10 worms per dish.
Measurements/observations
Mortality.
Evaluations
- Contact test: LC50 using the method of Litchfield & Wilcoxon (1949). The LC50 values are reported as ug of test chemical cm-2 of filter paper.
- EEC aritifical soil test: LC50 using the method of Litchfield & Wilcoxon (1949). The LC50 values are reported as mg chemical/kg dry wt of artificial soil.
[ref. ID; 7163]
Test system
Contact test (2 days) and soil test (14 days)
Strains
Toxicants
Carbaryl,
1,2-Dichloropropane,
Dimethyl phthalate,
Diphenylnitrosamine,
Fluorene,
Nitrobenzene,
4-Nitrophenol,
1,2,4-Trichlorobenzene,
2,4,6-Trichlorophenol.
Test design
Standard protocols.
Measurements/observations
Mortality.
Evaluations
LC50 and Weibull function.