Acrobeloides
- Acrobeloides buetschlii
- Acrobeloides nanus
[ref. ID; 1276]
Test system
Acute toxicity
Strains
Samples were taken near Wageningen, The Netherlands.
Toxicants
Cadmium chloride (CdCl2), Pentachlorophenol
Test design
LC50-tests were performed in water containing a defined mixture of minerals with concentrations resembling those found in interstitial water of sandy forest soils (K+: 0.1 mmol/L, Na+: 0.2 mmol/L, Ca2+: 0.35 mmol/L, Mg2+: 0.3 mmol/L, NH4+: 0.3 mmol/L, NO3-: 1.7 mmol/L, Cl-: 0.3 mmol/L) (Schouten and Van der Brugge 1989). pH 6.0+/-0.1.
Two replicates of the tests were carried out in multi-dishes (Greiner, 24 compartment plate, no. 662160) with lid and sealed with parafilm to minimize volatilization. Each compartment was filled with 0.9 ml of water containing toxicant. Samples of 0.1 ml of the multi-species suspension, containing 10-50 adult individuals of each species, were taken and suspended in each compartment. Temperature 20+/-0.1 degrees C in dark.
Measurements/observations
Mortality (24, 48, 72 and 96 hr).
Evaluations
LC50 according to the trimmed Spearmann-Karber method (Hamilton et al. 1977).
[ref. ID; 5915]
Test system
Strain
Toxicants
Carbofuran, Aroclor 1254
Temperature
27 degrees C
Test design/concentrations
The surface of 5 ml of sterile PDA medium (20 g potato dextrose agar per litre water) in 5-cm-diameter petri dishes was treated with 0.3 ml of the toxicants solution. The amount of chemical applied to the agar surface (area 19.6 cm2) thus ranged from 7.5 ug to 60 ug per petri dish. 20 active female nematodes (food: Micrococcus luteus (Schroeter, 1872) Cohn, 1872).
Measurements/observations
Body size and weight.