Ref ID : 972
Hellung-Larsen and Inger Lyhne; Effect of Shaking on the Growth of Diluted Cultures of Tetrahymena. J.Protozool. 39(2):345-349, 1992
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Cultures of Tetrahymena pyriformis, T. thermophila and T. pigmentosa have been studied with regard to growth rates in shaken and unshaken flasks. In the standard medium, a minimum doubling time of 170 min was obtained for T. pyriformis at 28 degrees C in the unshaken cultures. If the depth of the medium was less than 1 cm, the gyratoric shaking increased the doubling time to 340 min. The effect of shaking could be reduced by the addition of dextrane. Cells subjected to shaking were observed in different media and at different growth temperatures. If cultures were inoculated with 10E4 cells/ml or more, the effect of shaking was absent. However, with inoculates of 10E3 or 10E2 cells/ml, the doubling times for T. pyriformis increased to 240 and 275 min, respectively. Periods of 2 min shaking followed by rest for 60 min could not induce an effect.