Ref ID : 724
K. Plasota, M. Plasota, and W.J.H. Kunicki-Goldfinger; Methods for obtaining an axenic culture of Habrotrocha rosa Donner 1949. Hydrobiologia 73:37-38, 1980
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A culture of the bacterivorous rotifer H. rosa was separated from other Eucaryota in activated sludge and then purified by passages from most representatives of the bacterial microflora. A fully axenic culture was finally obtained by use of a lysing buffer and certain antibiotics.