Main Content

The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 349

Edward Gobel, Ruth Riessner, and Peter Pohl; [Influence of DCMU on the Formation of Lipids and Fatty acids, and on the Ultrastructure of Euglena gracilis]. Z.Naturforsch. 31c:687-692, 1976

Reprint

In File

Notes

Euglena gracilis Klebs (strain Z) was grown heterotrophically in the dark for 6 days. Subsequently, the cells were grown photoautotrophically under white fluorescent light with varying amounts of 3-(3,4-dichlorophenyl)-1,1-dimethyl urea (DCMU) (1.7x10E-9 - 1.0x10E-5 mol/l) added to the nutrient medium. Under non-photosynthetic conditions (addition of 10E-6 - 10E-5 mol DCMU/l), the cells still produced chloroplast structures and a considerable percentage of compounds typical for chloroplasts, such as galactolipids (40-50%), chlorophylls (40%), and poly-unsaturated C16 and C18 fatty acids (16:2, 16:3, 16:4, 18:2, 18:3) (65%). The results indicate that under these conditions the above compounds are synthesized from carbon sources outside the chloroplasts.