Ref ID : 103
Teshima Shinichi, Kanazawa Akio, Kamezaki Naoki, and Hirata Hachiro; Sterols of the Rotifer. Bull.Jap.Soc.Sci.Fisheries 45(12):1495-1501, 1979
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The sterol components of the rotifer, Brachionus plicatilis, the marine type of Chlorella sp., Chlorella saccharophila, and the baker's yeast, Saccharomyces cerevisie, were investigated by using combined gas-liquid chromatography and mass spectrometry. The rotifer contained 24-methylcholesta-7, 22-dienol as a major sterol (61% of total sterols), and other sterols such as cholesterol, cholestanol, 24-methylcholesta-5, 22E-dienol, 24-methylcholest-22-enol, cholest-7-enol, 24-ethylcholesta-5, 22-dienol, 24-methylcholest-7-enol, 24-methylenecholest-7-enol, E-24-ethylidenecholest-7-enol, and Z-24-ethylidenecholest-7-enol. The Chlorella and baker's yeast used for the diets of rotifer contained mainly cholesterol from acetate-1-[14C] but not C28- and C29-sterols. Considering these data, we suspect that the sterols except C27-sterols occurring in the rotifer probably originate from dietary sources.