Ref ID : 7802
Ian S. Zagon; Carchesium polypinum: Cytostructure after protargol silver deposition. Trans.Amer.Micros.Soc. 89(3):450-468, 1970
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A modified method of Bodian protargol silver staining was applied to a peritrich, Carchesium polypinum (Ciliata, Peritrichia, Peritrichida, Sessilina). Several of these specimens were examined directly by transmission electron microscopy in order to determine the selectivity of the protargol stain. In this method gold toning was mandatory for a satisfactory preparation. Stain deposition was highly selective with kinetosomes, cilia, nuclear material, and myonemes having a definite affinity. Striae of the somatic cell appearing under light optics were non-staining epiplasmic peaks, while alveoli surrounding these peaks contained the infundibular myoneme. Infundibular reticulated fiber, the outer fibers in the stalk, and the peripheral regions surrounding food vacuoles had an affinity for protargol at the electron microscope level of resolution, but were not visible with the light microscope. A discussion of protargol selectivity is also presented.