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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 7713

Jean-Claude Esteve; [Calcium, Calmoduline, Sites Glucidiques et Ingestion chez le Cilie Gymnostome Dileptus sp.]. Protistologica XX(1):15-25, 1984

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The use of prefixed cells allowed to ascertain some aspects of the regulation of ingestion in the Ciliate Dileptus sp. Adherence to the cytostomal lip seemed to be a prerequisite for phagocytosis; some membrane determinants, reduced by proteolytic treatment, in the predator as in the prey, are necessary. The whole process is calcium-dependent and a calmodulin is probably involved in the distribution of this cation since trifluoperazine is a potent inhibitor. As concanavalin A blocked ingestion, glucose and/or mannose residues could be involved in the regulation of phagocytosis in this Ciliate. At the ultrastructural level, with a still limited study, some peculiarities, known for others ciliated predators, were also found: a local differenciation of the glycocalyx, the interaction between cilia of the prey and the predator membrane and some fibrillar links between cytostomal vesicles and microtubules of transverse fibres.