Ref ID : 7298
Thomas K. Sawyer, Thomas A. Nerad, Earl J. Lewis, and Shawn M. McLaughlin; Acanthamoeba stevensoni N. Sp. (Protozoa: Amoebida) from Sewage-Contaminated Shellfish Beds in Raritan Bay, New York. J.Eukaryot.Microbiol. 40(6):742-746, 1993
Reprint
In File
Notes
Marine sediments from 12 shallow water stations in Raritan Bay, New York were tested for the presence of Acanthamoeba. Eight stations were positive for one or more species of Acanthamoeba, A. castellanii, A. comandoni, A. hatchetti, A. lenticulata, A. polyphaga, A. rhysodes, and Acanthamoeba spp. Isolates that grew at 38-40 degrees C were found at four stations (A. comandoni, A. lenticulata, and two unidentified strains). The two unknown strains were characterized on the basis of morphological features, isoenzyme profiles, and mouse pathogenicity tests. One of the two strains was determined to be a new species and is designated herein as Acanthamoeba stevensoni n. sp. ATCC 50388. Mature cysts were most similar to those of morphological Group II of Pussard & Pons (1977). Acanthamoeba stevensoni n. sp. was isolated from inshore coastal sediments where seawater ranged from 20-30 0/00 (ppt.). The sediments supported commercially valuable populations of hard clams, Mercenaria mercenaria, that required depuration prior to sale because of contamination by sewage-associated bacteria.