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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 7065

Willem H. De Smet; Preparation of rotifer trophi for light and scanning electron microscopy. Hydrobiologia 387/388:117-121, 1998

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The methods to prepare rotifer trophi for light and scanning electron microscopy are reviewed, and the rapid method used by the author is described. Rotifers are dissolved in a minimal amount of sodium hypochlorite solution on a coverslip, and serially rinsed in distilled water. The entire procedure is done under a microscope using micropipettes.