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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 6906

P. Walsh, C. El Adlouni, D. Nadeau, M. Fournier, D. Coderre, and G.G. Poirier; DNA Adducts in Earthworms exposed to a Contaminated Soil. Soil Biol.Biochem. 29(3/4):721-724, 1997

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DNA adducts were quantified by [32P]-postlabelling on the earthworm Eisenia fetida after an exposure to a polycyclic aromatic hydrocarbon (PAH)-contaminated soil sample. The pattern of adduct spots found on the chromatograms are consistent with the presence of bulky DNA adducts originating from PAH metabolites. After 1 or 2 weeks of exposure, amounts of DNA adducts in exposed worm (34.4 and 31.3 adducts per 10E9 nucleotides, respectively) were higher than those found in the unexposed control worms (16.5 adducts per 10E9 nucleotides).