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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 4555

Thomas Urbig, Rudiger Schulz, and Horst Senger; Inactivation and Reactivation of the Hydrogenases of the Green Algae Scenedesmus obliquus and Chlamydomonas reinhardtii. Z.Naturforsch. 48c:41-45, 1993

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The hydrogenases of the green algae Scenedesmus obliquus and Chlamydomonas reinhardtii were activated under anaerobic conditions. Exposure of whole cells and cell-free homogenates to air lead to a complete inactivation of the hydogenases. The inactivation in whole cells of Scenedesmus is faster than the inactivation of the cell-free homogenate. Inactivation of the hydrogenases could be reversed by anaerobic readaptation in whole cells. The inactivation of the hydrogenates in homogenates seems to be irreversible. Neither the removal of oxygen nor the addition of ATP, NAD(P)H, sodium dithionite, dithiothreitol, ferredoxin and thioredoxin to homogenates facilitated the reactivation of the hydrogenase. The occurrence of a hydrogenase regulating factor is discussed.