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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 4295

Anju Agrawal, V.C. Pandey, S. Kumar, and P. Sagar; Secretion of Acid Phosphatase by Axenic Entamoeba histolytica NIH-200 and Properties of the Extracellular Enzyme. J.Protozool. 36(1):90-92, 1989

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Entamoeba histolytica (NIH-200) secreted large amounts of acid phosphatase in its external environment when grown axenically in modified TPS-II medium. Fractionation by DEAE-cellulose chromatography of the precipitate obtained from the cell-free medium at 60% ammonium sulfate saturation yielded 3 distinct peaks of enzyme activity. The enzyme in all the peaks showed resistance to tartrate but was inhibited by fluoride, cupric chloride, ethylene diamine-tetra acetic acid, ammonium molybdate and cysteine; however, enzyme associated with different peaks differed in its polyacrylamide gel electrophoretic profiles and behavior towards concanavalin A.