Ref ID : 4255
Peter Suhr-Jessen; Constitutive and Induced Excretion of Polypeptides in Tetrahymena thermophila. J.Protozool. 34(1):54-57, 1987
Reprint
In File
Notes
Polypeptides normally excreted to growth or starvation media were revealed using O'Farrell gel-electrophoresis and silver-staining. The major polypeptides detected in conditioned media were either constitutive, growth specific, starvation specific, or high-Tris specific. The majority of the excreted polypeptides could be released from the cells by a hypo-osomotic shock, possibly resulting in membrane leakage, but also mucocysts and/or plasma membrane-associated polypeptides were detected in the conditioned media.