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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 4215

Simone Eperon and Gerard De Haller; Effect of Cytochalasin B on the Cytokinesis of Thuricola folliculata (Ciliophora, Peritrichida). J.Protozool. 33(2):243-245, 1986

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Binary fission of the sessiline loricate peritrich Thuricola folliculata was examined by light microscopy. Cytokinesis, which occurs in the oral-aboral median plane, is 35-40 min in duration. Cytochalasin B (CB) was used at final concentrations of 50-100 µg/ml in dimetyl sulfoxide (DMSO). A transition point occurs at about 20 min after the beginning of binary fission and about 10 min before cytokinesis. In most cases, 50 µg/ml and 100 µg/ml CB added to cells before the transition point resulted in delays of cytokinesis which were significant compared to DMSO controls; sometimes, cytokinesis was blocked completely. If added after the transition point, CB had no effect on cells.