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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 4131

Norman E. Williams; Localizing Surface-Related Proteins in Ciliates by Indirect Immunofluorescence Microscopy. J.Protozool. 31(3):492-494, 1984

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Results obtained in immunofluorescence localization studies involving three antisera, six species of ciliates, and a variety of fixation procedures suggest that superior results can often be obtained by fixing cells in 35-70% ethanol. Formaldehyde fixation appeared to induce redistributions of epiplasmic proteins surface antigens which were not observed in ethanol-fixed cells. In addition, background fluorescence was significantly lower in ethanol-fixed cells than it was in cells fixed in aldehydes.