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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 3932

T.E. Shehata and E.S. Kempner; Synchronization of Division in Vitamin B12-Starved Euglena gracilis. J.Protozool. 26(4):626-630, 1979

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Vitamin B12 deficiency arrests cells division in Euglena gracilis. B12 starvation for short periods made it possible to induce synchronous growth by addition of the vitamin. Culture conditions were established to optimize replenishment synchrony. The DNA content of E. gracilis in steady state culture and vitamin B12 deficiency culture was measured by flow cytofluormetry and was consistent with colorimetric determinations. The cell volume and DNA distribution of E. gracilis in synchronous culture were analyzed and the sequential changes during the division cycle were computed. Synchronous culture permits definitive studies of shifts in cell volume and DNA distribution, in which the biochemical events required of cell division presumably synchronized.