Ref ID : 3840
Jeffrey J. Seilhamer and Thomas J. Byers; Mutants of Acanthamoeba castellanii Resistant to Erythromycin, Chloramphenicol, and Oligomycin. J.Protozool. 25(4):486-489, 1978
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Cell lines of Acanthamoeba castellanii resistant to erythromycin (Ery), Chloramphenicol (Cap), and oligomycin (Oli) have been isolated. These may be the first such mutants for A. castellanii. These mutants have been phenotypically stable for 2 years, surviving storage and vegetative multiplication in the absence of drugs. Resistance was specific for each drug, but double mutants (e.g. Ery Cap) were obtained by stepwise selection. Mutant frequencies were determined in multiwell plates; <10 colony forming units (CFU/10E5 amebas were observed in wild-type populations 12 days after incubation in 500 µg Ery/ml, 2.5 mg Cap/ml, or 15 µg Oli/ml. After 30 days, averages of 100 CFU/10E5 amebas were observed in Ery and Cap, whereas, frequencies for Oli remained unchanged. Frequencies of Ery and Cap were consistent with rates of recovery from these drugs in batch cultures. We were unable to obtain spontaneous mutants resistant to cycloheximide, emetine, 5-fluorodeoxyuridine, or ethidium bromide. Ery, Cap and Oli could be mitochondrial mutants.