本文へスキップ


Microbial Culture Collection

Since 1983, culture strains of microalgae and protists have been maintained in the Microbial Culture Collection (NIES-Collection) for the purpose of research and monitoring on biodiversity and ecosystem conservation. At present (2017), about 3,700 strains are maintained. Among those, 2,728 strains, including various taxa, model organisms, type/authentic strains, and strains related with environmental issues, are open for distribution.

Microalgal strains have been used for both basic and applied research in addition to educational purposes and, more recently, for biorefinery and biomass research aiming to produce useful biomaterials and biomass.

The Collection also accepts the deposition of strains that are important for both basic and applied science. Not only is this useful for routine collection efforts, we have also achieved the development of efficient management system and improved quality of strains.
Furthermore, in order to expand usage of NIES-Collection, we have tried to accumulate additional information for each strain (DNA barcode and genome information, publication, and biochemical information) and updated contents of our official web pages.

Visit NIES Collection website



 
Two-thirds of algal strains in the NIES-Collection are maintained by subculture under each appropriate condition. They are regularly checked cell and growth state and transferred to new media.
 
Cryopreservation technique is applicable to certain groups of algae, resulting in about 1/3 of strains being frozen in liquid nitrogen.
 
Culture strains of charophytes regenerated from oospores in bottom sediment of Lake Kasumigaura.


Sample database of endangered species

A list of endangered algal species preserved in the NIES-collection can be searched and obtained from the endangered species sample database (released on July 5, 2012/Japanese version only).



Ex-situ Conservation of Endangered Algae

Various Japanese endangered algae are also preserved as culture strains at the Microbial Culture Collection facility. They include 14 species, 270 strains of freshwater red algae and 28 species, 100 strains of charophytes.

 

A freshwater red alga, Nemalionopsis tortuosa, growing on a stone in a stream.

 

A charophytes, Nitella pseudoflabellata, growing on the shore of an irrigation pond, making distinctively dominant community in particular water depth.

(KAWACHI Masanobu, Biodiversity Resource Conservation Section)